Elution Buffer In Mining Process

Repeat step 2.23, then centrifuge once more at 6,000 g for 30 s to remove residual buffer pe.Transfer the column to a new 1.5-ml microcentrifuge tube and add 100 l of elution buffer eb 10 mm triscl, ph 8.5 to the center of the column membrane.Incubate at rt for 10 min and centrifuge at 6,000 g for 1 min to elute the du-ssdna.